Multi-omics profiling of the cold tolerant Monoraphidium minutum 26B-AM in response to abiotic stress

Microalgae that are of interest for biofuel production must be able to tolerate environmental changes occur in outdoor cultivation systems. While algal cultures may experience daily temperature fluctuations and seasonal changes, the underlying mechanisms control regulate physiological responses adaptation pressures largely unknown. Systems-level characterization enabled by functional genomics can help identify biochemical pathways promote stability productivity algae various conditions. Monoraphidium minutum 26B-AM, a freshwater green microalga, was identified as top performer biomass winter season screens. We sequenced genome M. 26B-AM applied our multi-omics pipeline profile this high potential strain under salt cold perturbations. Through comparative analysis, including other class Chlorophyceae, we gene families unique genus Monoraphidium, desaturase has been linked tolerance plants. observed osmolytes, such trehalose, proline betaine, accumulate stress, coinciding with upregulation genes involved biosynthesis these metabolites. From annotation, reconstructed metabolic model provide detailed map used simulate growth reaction fluxes. This analysis provides foundation explore applications, guides engineering, expands understanding regulatory